If the player harvests more than one Little Sister, Jack turns on the Little Sisters to harvest their ADAM.[15] Tenenbaum sadly narrates what occurs, condemning Jack and his actions. A submarine comes across the plane's wreckage and finds itself surrounded by bathyspheres containing Splicers, who attack the crew and take control of it.

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In 2002, the team had come up with a core gameplay mechanic idea based on three groups of forces; drones that would carry a desirable resource, protectors that would guard the drones, and harvesters that would attempt to take the resource from the drones; these would eventually bear out as the Little Sisters, Big Daddies, and Splicers in the final game, but at the time of the concept, there was no set theme.[28] They began working on creating a setting as to be able to pitch the idea to publishers.[28] A 2002 demonstration version was based on the Unreal Engine 2 for the Xbox.[27] This demonstration was primarily set aboard a space station overtaken with genetically mutated monsters; the main character was Carlos Cuello, a "cult deprogrammer"—a person charged with rescuing someone from a cult, and mentally and psychologically readjusting that person to a normal life.

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Levine confirmed in March 2013 that the film had been officially canceled. Levine stated that after Warner's Watchmen film in 2009 did not do as well as the studio expected, they had concerns with the $200 million budget that Verbinski had for the BioShock film. They asked him to consider doing the film on a smaller $80 million budget, but Verbinski did not want to accept this.[206] In February 2017, Verbinski said that his crew was about eight weeks from starting filming,[207] with plans for many elaborate sets given that the setting of Rapture could not be something easily shot on existing locations, requiring the $200 million budget. Verbinski was anticipating on releasing the film with an R-rating when Universal approached him about changing the film's direction.

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In December 2007, a common patch was released for both the Xbox 360 and Windows version. The patch included additional content such as new Plasmids, new achievements for the Xbox 360 version, and additional graphics settings to address some of the field-of-view issues identified by players.

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